Part:BBa_I725013:Design

Butyryl CoA dehyrogenase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 247
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA.

Source

C. acetobutylicum genom. CAC_2712